Fermentation Quality and Bacterial Ecology of Grass Silage Modulated by Additive Treatments, Extent of Compaction and Soil Contamination

New technologies related to the identification of bacterial communities in fresh forage and silage may give valuable detailed information on best practices produce animal feeds. The objective was evaluate how management conditions during making manipulate profile fermentation quality grass silages. Silages were prepared from mixed timothy meadow fescue using two compaction levels. As an additional treatment contaminated with soil feces prior tight compaction. Four additive treatments different modes action applied: control without additive, formic acid-based homofermentative lactic acid bacteria salt-based additive. After 93 days silos opened, samples taken routinely analyzed. DNA extraction carried out PCR amplification 16S rRNA gene V4 region performed universal primers. pH higher for loose than non-contaminated Great shift observed profiles material towards silage. Lactobacillus genus barely found relative abundance but became predominant final along Sphingomonas genus. Use additives improved modified ensiled under conditions.